Phase-contrast micrograph of ClonalStem CB-ECFCs at confluence.
In stock
Human Cord Blood-Derived Endothelial Colony-Forming Cells (ECFC)
SKU: CS-ECFC-HCB-1M · 1 × 10⁶ cells / vial · Cryopreserved
USD 1,199
High-fidelity Human Endothelial Colony-Forming Cells (ECFCs) derived from cord blood. True endothelial progenitors with high proliferative potential and de novo vessel formation capability.
- High proliferative potential — >100 population doublings without senescence.
- True vasculogenesis — spontaneous lumenized networks in 3D matrices.
- Defined purity — CD31+, CD144+, NRP1+, CD45−.
Media & handling: We recommend EGM-2 (Lonza) or an equivalent complete endothelial growth medium with 5–10% FBS, on fibronectin- or collagen-coated vessels. Medium and coating reagents are not supplied with the cells.
Shipping: Typical lead time is 3–5 business days from order confirmation. Overnight dry-ice delivery within the US; international shipping is available — contact us for a country-specific lead time and any import documentation.
For Research Use Only
Not approved for use in human subjects or for clinical, diagnostic, or therapeutic use in animals or humans.
Orders are subject to our .
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Description
Human Cord Blood Endothelial Colony-Forming Cells (ECFCs) by ClonalStem Inc. are a rare, highly proliferative subtype of endothelial progenitor cells derived from human umbilical cord blood. Unlike mature endothelial cells (such as HUVECs) or Early Outgrowth Cells (EOCs), ECFCs demonstrate true "stemness" — possessing high clonal proliferative potential and the intrinsic ability to self-assemble into functional, perfusable vascular networks in vivo and in vitro.
These "Gold Standard" endothelial cells are isolated via ClonalStem's rigorous clonal selection to ensure a high-fidelity population distinct from hematopoietic contaminants. They are the ideal reference standard for researchers requiring robust vessel formation, long-term expansion, and reproducibility in vascular biology.
These cells are critical for advanced research in cardiovascular disease, ischemia/reperfusion injury, blood-brain barrier (BBB) modeling, and tumor angiogenesis. ClonalStem Inc. delivers a stable, reproducible platform for drug screening and tissue engineering where consistent vascularization is the limiting factor.
Also known as: ECFCs, EFCs, Endothelial Progenitor Cells, EPCs, Blood Outgrowth Endothelial Cells, BOECs, Cord Blood Endothelial Cells, Colony Forming Endothelial Cells.
Specifications
| Catalog Number | CS-ECFC-HCB-1M |
| Species | Human |
| Tissue | Umbilical Cord Blood |
| Sex | Female |
| Quantity | 1 × 10⁶ cells / vial (1 mL) |
| Format | Cryopreserved, single-donor |
| Country of Manufacture | USA |
| Lot Number | Included with shipment |
| Recommended Medium | EGM-2 (Lonza) or equivalent — not supplied |
| Storage | Transfer immediately from dry ice to liquid nitrogen upon arrival. |
| Shipping | Dry ice, overnight delivery · 3–5 business day lead time · international available |
Quality Control
These cells are characterized by flow cytometry for CD31, CD144 (VE-Cadherin), and CD304 (NRP1), and are confirmed negative for CD45.
- Negative for Mycoplasma, bacteria, fungi, and yeast.
- Human viral pathogens HIV-1, HIV-2, HTLV-1, HTLV-2, HepA, HepB, and HepC are not detected.
- Isolated from single-donor umbilical cord blood.
Passage & Expansion
Passage number is commonly used to describe cell proliferation capacity, but the true capacity depends on the total number of cell divisions — the population doubling number (PDN) — that a cell type can undergo. Cells passaged at low confluence will require many more doublings to reach confluence, which can yield a low passage number but a population with limited remaining division capacity.
ClonalStem expresses future cell-division capacity as the population doubling number (PDN) remaining after thaw, and current division status as the population doubling level (PDL) reached before cryopreservation. Our early-passage CB-ECFCs are characterized to support extensive expansion under recommended culture conditions.
Product Use
This cell product is intended for laboratory research purposes only. Not approved for use in human subjects or for clinical, diagnostic, or therapeutic use in animals or humans.
This product is provided "AS IS". The customer assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the product, and agrees that any activity undertaken with the product (and any progeny or modifications) will comply with all applicable laws, regulations, and guidelines. Any proposed commercial use is prohibited without a license from ClonalStem Inc.